Below Are the Steps Involved in the RNA-Seq Process

Multiple Choice

Below are the steps involved in the RNA-seq process. Place these steps in the CORRECT order. 1. Conversion of the RNA to cDNA
2) Fragmentation and preparation of the cDNAs for sequencing
3) Isolation of the RNA molecules of interest from cells
4) Assembly of the sequencing reads into RNA transcripts
5) Sequencing of the cDNA

A) 3, 1, 2, 5, 4
B) 1, 2, 4, 5, 3
C) 3, 4, 1, 2, 5
D) 1, 5, 4, 3, 2
E) 1, 5, 2, 3, 4