Choose the CORRECT Order of Procedure in Performing Chromatin Immunoprecipitation

Multiple Choice

Choose the CORRECT order of procedure in performing chromatin immunoprecipitation (ChIP) . 1. DNA sequencing and fragment identification
2) Removal of crosslinking and separation of DNA and proteins
3) Crosslinking proteins and chromosomes via UV
4) Antibody incubation for immunoprecipitation
5) Protein degradation via proteases
6) Cell lysis and chromosome fragmentation

A) 1, 3, 2, 4, 5, 6
B) 2, 6, 5, 1, 3, 4
C) 4, 3, 2, 5, 1, 5
D) 3, 6, 4, 2, 5, 1
E) 5, 1, 3, 6, 4, 2