Use the Following to Answer Questions
an Auxotrophic E

Multiple Choice

Use the following to answer questions
An auxotrophic E. coli strain requires adenine to grow because of a mutation in a gene for an adenine synthesis enzyme. The following shows part of the wild-type and mutant alleles of the gene, including the start codon. The bottom strand is the template for transcription. $$\begin{array} { c c } \text { ade } 1 ^ { + } \text {wild-type allele } & \text { ade1 } ^ { - } \text {mutant allele } \\\text { 5'...TTATGGGCAAGATCCCA...3' } & \text { 5'....TTA TGGGCTAGATCCCA...3' } \\\text { 3'...AATACCCGTTCTAGGGT...5' } & \text { 3'...AATATGGGCTAGATCCCA...3' }\end{array}$$
-How does the mutation affect the enzyme?

A) The protein won't be synthesized and translation will stop at the third codon.
B) The protein will not be changed because the mutation did not change the amino acid.
C) The protein will be changed because the mutation altered the amino acid.
D) The protein will not be changed because the mRNA was not changed.