An in Vitro Transcription System That Contains a Bacterial Gene

Multiple Choice

An in vitro transcription system that contains a bacterial gene does not initiate transcription. What is one possible problem?

A) Histones that were on the DNA when it was isolated from E. coli are blocking access to the template.
B) There is a mutation in the inverted repeat sequence that prevents a hairpin secondary structure from forming.
C) There is a mutation at -10, where a promoter consensus sequence is located.
D) Rho factor has not been added.
E) TATA-binding protein (TBP) has not been added.