An in Vitro Transcription System Transcribes a Bacterial Gene but Terminates

Multiple Choice

An in vitro transcription system transcribes a bacterial gene but terminates inefficiently. What is one possible problem?

A) There is a mutation in the -10 consensus sequence, which is required for efficient termination.
B) Rho factor has not been added.
C) Sigma factor has not been added.
D) A hairpin secondary structure has formed at the 3' end of the mRNA, interfering with termination.
E) Histones were added prematurely and interfered with termination.