Your Goal Is to Achieve a High Level of Transcription-A

Essay

Your goal is to achieve a high level of transcription-a level similar to its in vivo levels-of a eukaryotic gene in an in vitro transcription system. You add the DNA template for this gene, which contains the following, to the in vitro transcription system: a TATA box sequence located -25 to -30 bp upstream of the transcription start site, the sequence for the gene, and terminator sequences. How would you interpret the result if you do not get high levels of RNA transcription, and what corrective measures would you take to achieve your goal?