While Working on Drosophila, You Find a New Mutant Strain

Multiple Choice

While working on Drosophila, you find a new mutant strain with an abnormal histone H3 gene. This novel histone mutant is predicted to cause the nucleosomes to bind an extra 15 bp of DNA compared to wild type. If you digest isolated chromatin with a nuclease to release the core nucleosomes, what size DNA fragments would you expect from wild-type and mutant flies?

A) Approximately 50 bp is known to bind to the normal core nucleosome, so the additional 15 bp binding to H3 would give a rise to ~65 bp.
B) Approximately 100 bp is known to bind to the normal core nucleosome, so the additional 15 bp binding to H3 would give a rise to ~115 bp.
C) Approximately 125 bp is known to bind to the normal core nucleosome, so the additional 15 bp binding to H3 would give a rise to ~140 bp.
D) Approximately 145 bp is known to bind to the normal core nucleosome, so the additional 15 bp binding to H3 would give a rise to ~160 bp.
E) The size of the DNA fragments cannot be determined.