The Acceptability for FISH Analysis of Cells on a Prepared

Multiple Choice

The acceptability for FISH analysis of cells on a prepared slide is most influenced by

A) temperature and humidity while fixed cells are drying on the slide.
B) pH of the fixative solution.
C) ionic strength of the fixative solution.
D) detergent used to permeabilize the cell and nuclear membranes.
E) detergent used to rupture the cell and nuclear membranes.