Multiple Choice
The three basic steps of polymerase chain reaction (PCR) are denaturation, primer annealing, and primer extension. Which specification listed below is correct?
A) Primer design-oligonucleotide of 15- 30 base pairs
B) Primer extension-10 minutes during each cycle
C) Primer annealing-95° C for 1 minute
D) Denaturation-50° C for 5 minutes
E) Primer extension-37° C when Taq polymerase is used
Correct Answer:
Verified
Correct Answer:
Verified
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