A Flow Cytometry Analysis Was Set Up as Follows

Multiple Choice

A flow cytometry analysis was set up as follows. How can T helper cells be identified? Analysis of forward and side laser light scatter anti- CD3 labeled with green fluorochrome anti- CD4 labeled with red fluorochrome
Anti- CD8 labeled with yellow fluorochrome anti- CD19 labeled with blue fluorochrome

A) Select cells with the highest forward and lowest side scatter plus red and green fluorescence.
B) Select cells with the lowest forward and highest side scatter plus red fluorescence only.
C) Select cells with the lowest forward and side scatter plus yellow and green fluorescence.
D) Select cells with the lowest forward and side scatter plus red and blue fluorescence.
E) Select cells with the lowest forward and side scatter plus red and green fluorescence.