All of the Following Are TRUE Regarding Specimen Handling for Flow

Multiple Choice

All of the following are TRUE regarding specimen handling for flow cytometry except

A) ammonium chloride is used to lyse granulocytes when mononuclear cells need to be analyzed.
B) tissues must be homogenized into single cell suspensions.
C) a minimum number of cells must be present for analysis to be accurate.
D) specimens must be processed promptly to insure cell viability.
E) cell surface antigens must be stabilized with paraformaldehyde.