Each of the Following Is a Step in Screening Organ

Multiple Choice

Each of the following is a step in screening organ recipient serum for HLA antibodies except

A) Step 1: A panel of latex beads is coated with HLA antigens.
B) Step 2: Recipient serum is mixed with each HLA antigen.
C) Step 5: The latex beads are put in the flow cytometer and analyzed for agglutination based on increased forward scatter of agglutinated particles.
D) Step 4: Fluorochrome- labeled antihuman globulin is mixed with the latex beads.
E) Step 3: After serum has incubated with the beads, they are washed to remove all excess serum.