Use the Following Information to Answer the Question

Multiple Choice

Use the following information to answer the question.
"The native structure of hemoglobin (Hb) comprises of two α and two β subunits, each of which carries a heme group. There appear to be no previous studies that report the in-vitro folding and assembly of Hb from highly unfolded α and β globin in a 'one-pot' reaction. One difficulty that has to be overcome for studies of this kind is the tendency of Hb to aggregate during refolding. This work demonstrates that denaturation of Hb in 40% acetonitrile at pH 10.0 is reversible." (J Am Soc Mass Spectrum 2007, 18, 8-16)
Hemoglobin, when subjected to 40% acetonitrile at pH 10.0, loses its quaternary structure, which means the ________.

A) four α and β polypeptides dissociate
B) peptide bonds between amino acids break
C) α and β polypeptides lose their 3-D structure
D) four α and β polypeptides dissociate, peptide bonds between amino acids, and α and β polypeptides lose their 3-D structure