Exposing Inner Mitochondrial Membranes to Ultrasonic Vibrations Will Disrupt the Membranes

Multiple Choice

Exposing inner mitochondrial membranes to ultrasonic vibrations will disrupt the membranes. However, the fragments will reseal "inside out." The little vesicles that result can still transfer electrons from NADH to oxygen and synthesize ATP. Which of the following statements about these inside-out membrane vesicles is true?

A) The inside of the vesicles will become acidic when NADH is added.
B) The inside of the vesicles will become alkaline when NADH is added.
C) ATP will be produced from ADP and ᵢ in the interior of the vesicle.
D) The vesicles will pump protons out of the interior of the vesicle to the exterior using energy from ATP hydrolysis.