You Grow a Barcoded Yeast Deletion Set at 4°C and Then

Multiple Choice

You grow a barcoded yeast deletion set at 4°C and then a copy of the same set at 25°C.Barcodes from the 4°C sample and 25°C are labeled with a green fluorochrome and a red fluorochrome,respectively.How will you identify genes required for growth specifically at cold temperatures?

A) separate the PCR products on an agarose gel and look for red fluorescent bands
B) separate the PCR products on an agarose gel and look for yellow fluorescent bands
C) hybridize the products to a microarray and look for red spots
D) hybridize the products to a microarray and look for green spots
E) separate the PCR products on an agarose gel and look for green fluorescent bands