Which of the Following Limits the Utility of Yeast Two-Hybrid

Multiple Choice

Which of the following limits the utility of yeast two-hybrid screens in identifying protein interactions?

A) Yeast two-hybrid screens can only be applied to bait proteins that are less than 20kDa.
B) Only two proteins can be tested for their ability to interact,per day.
C) Yeast two-hybrid screens can only find proteins that interact with transcription factors.
D) Fusion proteins must be affinity purified.
E) Bait proteins may not fold properly when fused to other proteins.