Multiple Choice
Genomic DNA is isolated from bacteria.After a long digestion with a 6-base cutting enzyme that recognizes the sequence GAATTC,the DNA is not cut.The genome is 4 Mb,so there should be approximately 1,000 sites in the DNA.What is the most likely explanation?
A) There are no Gs in the genome.
B) There are no As in the genome.
C) The bacterium methylated the DNA,because it produces the 6-base cutting enzyme.
D) All the recognition sites are in genes,and the enzyme will not cut in genes.
E) All the recognition sites are in promoters and regulatory sequences,and the enzyme cuts only in genes.
Correct Answer:
Verified
Correct Answer:
Verified
Q16: Probability of a 6-base restriction enzyme site
Q17: A genomic library is a<br>A) collection of
Q19: PCR is used to<br>A) generate DNA fragments
Q20: The genomic equivalent of a library is
Q22: A genomic library for an organism with
Q23: If DNA had 5 different bases,a restriction
Q24: Which choice is not a characteristic of
Q25: A restriction enzyme with a recognition site
Q26: The following image shows the products from
Q28: In gel electrophoresis of DNA, fragments move