Assume the Two E

Multiple Choice

Assume the two E.coli strains shown below are allowed to conjugate. Hfr: pro+, arg+, his+, lys+, met+, ampicillin-sensitive F-: pro-, arg-, his-, lys-, met-, ampicillin-resistant
What supplements would you add to glucose minimal salts agar to select for a recombinant cell that is lys+, arg+, amp-resistant?

A) ampicillin, proline, histidine, lysine
B) lysine, arginine
C) ampicillin, proline, histidine, methionine
D) proline, histidine, methionine
E) ampicillin, lysine, arginine