If You Were Setting Up an Experiment to Disprove Spontaneous

Multiple Choice

If you were setting up an experiment to disprove spontaneous generation in a liquid medium, which of the following would be essential to the experiment?

A) supplying the liquid with nutrients
B) starting with a liquid that contains microorganisms
C) adding antibiotics to the liquid
D) adding carbon dioxide to the liquid
E) using a sterile liquid and eliminating exposure to microorganisms