While Performing an ELISA, a Lab Technician Fails to Rinse

Multiple Choice

While performing an ELISA, a lab technician fails to rinse away unbound detection antibody from the wells before adding the substrate for the reporter enzyme. What is the likely outcome of this error?

A) All wells, including controls, will indicate a negative result.
B) All wells, including controls, will indicate a positive result.
C) The test results will not be affected.
D) The control wells will indicate a negative result, but the wells with the test sample will indicate a positive result.
E) The outcome cannot be determined from this information.