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Which Aspect of the Polymerase Chain Reaction (PCR) Is Mismatched

Question 27

Multiple Choice

Which aspect of the polymerase chain reaction (PCR) is mismatched with its description?


A) variable temperatures: the highest temperature required for double- stranded DNA separation, the lowest temperature to allow primers to anneal, and a midrange- temperature for DNA replication
B) Taq polymerase: a DNA- replication enzyme which functions at high temperatures
C) dNTPs: building blocks of DNA
D) template DNA: the DNA to be copied; must be present at a high concentration for the procedure to work
E) DNA primers: sequences specifically chosen to be complementary to the DNA which flanks a target gene or region of interest

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