A Researcher Is Annotating a Newly- Sequenced Chromosome That They

Multiple Choice

A researcher is annotating a newly- sequenced chromosome that they constructed from contigs sequenced from a sample of Antarctic ice. The cell/organism that the chromosome came from is not known, however it yielded a single circular chromosome. You are asked to run a BLAST search to help this researcher identify the most related organism in GenBank. What would be the best strategy?

A) Perform many careful one- by- one comparisons between the newly- discovered DNA sequence and species selected from GenBank, one at a time.
B) Look for an exact match to the whole chromosome.
C) Look for a nearly identical match to a very small part (20- 40 bp) of the chromosome from all known ice bacteria.
D) Use several ~1000bp portions of the DNA sequence to search for the closest matches from among the entire available database.
E) Compare the most unusual portion of this newly- discovered chromosome to al known animal genomes.