In Reverse Genetics, What Is the Correct Order in Which

Multiple Choice

In reverse genetics, what is the correct order in which the experimenter proceeds?

A) selection of mutants with a phenotype of interest and then mapping to locate the effected gene(s)
B) identification of a gene of interest followed by screening for mutations in that gene and studies of the phenotypes caused those mutations.
C) random bombardment of the DNA with a known mutagen, followed by observation of offspring for newly acquired traits
D) screening individuals by PCR/DNA sequencing to associate altered genes with the trait of interest
E) silencing the genes in question using RNAi, followed by mapping to locate the gene