A Gene, VTE4, from the Sunflower of Known Sequence and Well-

Multiple Choice

A gene, VTE4, from the sunflower of known sequence and well- understood transcriptional regulation, is available from another lab. The product of this gene enables sunflowers to to produce a significantly increased amount of vitamin E from a substrate produced in all plants. The plant Brassica napus is harvested for canola oil. To produce canola oil with increased vitamin E, how would you proceed?

A) Clone VTE4 in A. tumefaciens and use these bacteria to produce transgenic Brassica.
B) Use a yeast system to engineer yeasts that produce the vitamin, and get manufacturers to add this to the canola oil during bottling.
C) Introduce the gene and necessary cis elements into Brassica, along with a reporter gene, then measure the vitamin E produced.
D) Introduce VTE4 into tomato plants or another food that humans eat more than Brassica.
E) Select Brassica plants with the highest titer of the vitamin, isolate the genes responsible, and cause these genes to duplicate.