Northern Blot Analysis Is Performed on MRNA Isolated from E

Short Answer

Northern blot analysis is performed on mRNA isolated from E. coli after growth in a glucose- only medium (lane 1) or a lactose- only medium (lane 2). The blots are hybridized with a probe for a portion of the lacZ sequence. Indicate the expected results for the following bacterial genotypes using dark, light, or no shading to i full, low, or no expression, respectively.
a. I+ P+ O+ Z+ Y+
b. I+ P- O+ Z+ Y+
c. I- P+ O+ Z+ Y+
d. I+ P+ OC Z+ Y+
e. I+ P+ O+ Z+ Y+ and a mutation of the CAP binding site preventing CAP- cAMP binding