Hereditary Anemia Known as Sickle Cell Disease (SCD) Results from Inheritance

Multiple Choice

Hereditary anemia known as sickle cell disease (SCD) results from inheritance of a variant form of fi- globin protein (fiS) , rather than the wild- type fi- globin protein (fiA) . The fiS protein does not migrate as far as the fiA protein. Which of following does NOT explain why the gel electrophoresis lane containing the hemoglobin protein from the heterozygous (fiAfiS) individual has two protein bands?

A) The protein bands migrated different distances based solely on differences in molecular weight.
B) The different electrophoretic mobility of the two proteins was a result of differences in their molecular weight, charge, and/ or shape.
C) The band closer to the origin of migration contained fiS protein and the band farther from the origin of migration contained fiA protein.
D) The fiS protein has a lower electrophoretic mobility.
E) The fiA protein has a higher electrophoretic mobility.