Using the Techniques of Genetic Engineering, You Design a Cytoplasmic

Multiple Choice

Using the techniques of genetic engineering, you design a cytoplasmic protein you want to accumulate within the ER of yeast cells. To accomplish this goal, you need to

A) incorporate radioactive amino acids into the protein.
B) incorporate appropriate DNA sequence to create signal sequences into mature peptide.
C) do nothing; all proteins go through the ER.
D) incorporate appropriateDNA sequence to create signal sequences into the mature peptide.
E) incorporate appropriate mannose- 6- phosphate groups.