In the Sanger Method of DNA Sequencing, Incorporation of the Nucleotides

Multiple Choice

In the Sanger method of DNA sequencing, incorporation of the nucleotides stops because

A) the dideoxynucleotide analogs have no 2'- OH group to continue DNA synthesis.
B) the dideoxynucleotide analogs have no 3'- OH group to continue DNA synthesis.
C) the mixture is heated to 92°C to stop DNA synthesis at specific time intervals.
D) the dideoxynucleotide analogs are in greater concentration than the actual nucleotides.
E) the dideoxynucleotide analogs have no 5'- OH group to continue DNA synthesis.