Using the Techniques of Genetic Engineering, You Design a Cytoplasmic

Multiple Choice

Using the techniques of genetic engineering, you design a cytoplasmic protein you want to accumulate within the ER of yeast cells. To accomplish this goal, you need to

A) incorporate the appropriate DNA sequence with the proper signal.
B) incorporate the appropriate mannose- 6- phosphate groups.
C) do nothing; all proteins go through the ER.
D) incorporate the appropriate lipid groups into the lipoprotein.
E) incorporate radioactive amino acids into the protein.