A Mixture of Four Proteins X, Y, Z and N

Multiple Choice

A mixture of four proteins X, Y, Z and N is applied to a gel-filtration column. Given the information supplied, which will elute first? $\begin{array}{|c|c|}\hline \text { Protein } & \text { Molecular Weight g/mol) } \\\hline X & 35,000 \\\hline Y & 26,000 \\\hline Z & 146,000 \\\hline \mathrm{N} & 26,000 \\\hline\end{array}$

A) Z
B) Y and N elute together
C) X
D) cannot answer without information about the isoelectric point