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A Mixture of Two Proteins with the Same PI and Molecular

Question 9

Multiple Choice

A mixture of two proteins with the same pI and molecular weights (MW) of 10,000 and 15,000 daltons,respectively,are applied to a gel filtration column.What happens during elution?


A) Both elute together.
B) The one with MW of 10,000 elutes first.
C) The one with MW of 15,000 elutes first.
D) More information is needed about the specificity of the column matrix to tell what happens.

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