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What Is the Purpose of Treating a Protein with 2-Mercaptoethanol

Question 80

Multiple Choice

What is the purpose of treating a protein with 2-mercaptoethanol?


A) to hydrolyze the protein into its amino acids
B) to cleave the disulfide bonds
C) to derivatize any free sulfhydryl groups to prevent them from reforming disulfide bonds
D) to derivatize the N-terminal amino acid during the Edman degradation

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