In a FISH Experiment, What Would Happen If Unbound Probe

Multiple Choice

In a FISH experiment, what would happen if unbound probe was not washed off?

A) Nothing-it's not necessary to wash off the unbound probe and doing so just adds an extra step to the procedure.
B) You would get False-positive results in different areas where the probe hadn't actually bound, but it was still sitting there and lighting up.
C) Your FISH would be floating at the top of the tank due to the toxicity of the probe building up within them.
D) Nothing-the target nucleotide sequences are labeled, not the probe. Therefore, excess unbound probe wouldn't matter for the experiment.
E) You would get an intermediate color on the array because of the presence of both bound and unbound probes that fluoresce at different temperatures.