The Primer Used in Sanger (Dideoxy Chain Termination) Sequencing

Multiple Choice

The primer used in Sanger (dideoxy chain termination) sequencing

A) can have any random nucleotide sequence.
B) has a nucleotide sequence complementary to the 5' end or the 3' end of the region to be copied.
C) has a nucleotide sequence complementary to the 5' end of the region to be copied.
D) must have a sequence beginning and ending with the same nucleotide.
E) has a nucleotide sequence complementary to the 3' end of the region to be copied.