You Have Made a Speci?c DNA Probe That Will Bind

Multiple Choice

You have made a speci?c DNA probe that will bind to a key sequence on the DNA of Neisseria gonorrhoeae, allowing your company to market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens. However, upon testing it out against a known Neisseria gonorrhoeae culture, you ?nd that it does not work. Which of the following is a possible explanation for this negative result?

A) You forgot to incubate the test at the 75oC temperature required for hybridization.
B) You forgot to label the probe sequence with a reporter molecule.
C) You forgot to add the mRNA to the DNA probe sample.
D) You forgot to digest the probe with restriction endonucleases.