You Have Made a Smear of a Bacterial Culture and Have

Multiple Choice

You have made a smear of a bacterial culture and have performed the Gram stain on it. Looking at the organism under the microscope, you notice that the cells do not seem to be the dark blue- Purple of a gram-positive reaction, but instead are light purple. Your staining procedure was Performed correctly. What is your best explanation as to why the bacteria have stained this way?

A) The specimen did not undergo heat ?xation before staining; therefore, the primary dye does not stick properly to the wall.
B) These are mutant bacteria which cannot be stained like other bacteria.
C) There is something in the wall of the bacteria that has affected the uptake of the crystal violet into the cell wall, thereby staining it improperly.
D) The bacteria were taken from an inappropriate medium, and a chemical is interfering with proper staining of the cells.