Why Have Frozen-Hydrated Particles Largely Replaced Negatively Stained Specimens for High

Multiple Choice

Why have frozen-hydrated particles largely replaced negatively stained specimens for high resolution cryo-EM studies?

A) Frozen-hydrated particles are more likely to generate artifacts.
B) Frozen-hydrated particles are much less likely to generate artifacts.
C) Frozen-hydrated particles take positively charged stains more efficiently
D) Frozen-hydrated particles are much better suited for the study of external features within the particle's structure.