A Gene Sequence Is Cut at a Restriction Site and DNA

Multiple Choice

A gene sequence is cut at a restriction site and DNA polymerase is then used to make the single-stranded regions of the sticky ends into double-stranded DNA. The ends of the altered DNA are then ligated back together. What is the effect on the gene sequence?

A) Its amino acids remain constant
B) The reading frame of the gene is destroyed.
C) The gene is unchanged.
D) The gene is transposed.
E) The gene is inverted.