A Principal Problem with Inserting an Unmodified Mammalian Gene into a BAC

Multiple Choice

A principal problem with inserting an unmodified mammalian gene into a BAC, and then getting that gene expressed in bacteria, is that

A) prokaryotes use a different genetic code from that of eukaryotes.
B) bacteria translate polycistronic messages only.
C) bacteria cannot remove eukaryotic introns.
D) bacterial RNA polymerase cannot make RNA complementary to mammalian DNA.
E) bacterial DNA is not found in a membrane-bounded nucleus and is therefore incompatible with mammalian DNA.