Which of the Following Most Correctly Describes a Shotgun Technique

Multiple Choice

Which of the following most correctly describes a shotgun technique for sequencing a genome?

A) genetic mapping followed immediately by sequencing
B) physical mapping followed immediately by sequencing
C) cloning large genome fragments into very large vectors such as YACs, followed by sequencing
D) cloning several sizes of fragments into various size vectors, ordering the clones, and then sequencing them
E) cloning the whole genome directly, from one end to the other