A "Normal", Not Deionized Water Was Used in the Experiment

Multiple Choice

A "normal", not deionized water was used in the experiment that included an enzymatic reaction. The experiment did not work as expected. What is the MOST LIKELY reason for the failure?

A) The "normal" water used in the experiment contained ions that interacted with the enzyme activity.
B) Deionized water is usually warmer, and the experimenter forgot to heat up the water from the "normal" faucet.
C) Deionized water most likely gave the false positive result due to lack of ions.
D) "Normal" water gave false negative results due to lack of necessary ions that deionized water has.