Which of the Following Is NOT Part of Initiation of Transcription

Multiple Choice

Which of the following is NOT part of initiation of transcription?

A) -10 and -35 sequences
B) binding of RNA polymerase to the beginning of the gene
C) melting of the helix
D) binding of sigma factor to promoter followed by binding of core RNA polymerase
E) base pairing of the first nucleotide of the RNA