Multiple Choice
His₆-fusion-tagged proteins can be recovered from affinity chromatography columns because the histidine tag tightly binds to beads coated with ________ ions. The elution buffer contains ________, which has stronger affinity for these ions.
A) copper; sulfate
B) magnesium; EDTA
C) iron; citrate
D) calcium; EGTA
E) nickel; imidazole
Correct Answer:
Verified
Correct Answer:
Verified
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