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At Which Time Period(s) During PCR Thermocycling Is/are Hottest in Temperature

Question 31

Multiple Choice

At which time period(s) during PCR thermocycling is/are hottest in temperature?


A) during DNA denaturation
B) during primer annealing
C) during primer extension/elongation
D) Both the first and last cycles are hotter in temperature than all other cycles.

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