The Colonies of Interest in This Case Are the Blue-Green

Multiple Choice

The colonies of interest in this case are the blue-green presumptive Shigella colonies. However, growth on hektoen enteric agar is not sufficient for definitive identification. Further tests are needed. First, the lab technicians need to prepare larger numbers of Shigella from your patient's stool sample, and then they need to ensure that they are culturing a single species. The methods used to do this are ________, followed by ________. 

A) streak plating; pour plates
B) enrichment culture; Gram staining
C) Gram staining; enrichment culture
D) Gram staining; capsule staining
E) enrichment culture; streak plating