What Would the Outcome Be If You Forgot to Do

Multiple Choice

What would the outcome be if you forgot to do the washing step when carrying out FISH on a sample containing bacteria?

A) Nothing. The washing step is recommended but is not necessary and can be omitted.
B) Unbound probe would remain on the slide and would fluoresce, making it impossible to see individual cells.
C) The unbound probe would anneal strongly to the glass slide in random patterns that would look like bacteria. 
D) The unbound probe would cause lysis of the target bacterial cells, destroying their nuclei and mitochondria.
E) Nothing. Unbound probe will not fluoresce, so even if it is present, it will not affect the results.