A Manual Hemoglobin Is Determined on a Patient,and the Dilution

Multiple Choice

A manual hemoglobin is determined on a patient,and the dilution still appears turbid after letting it sit for 15 minutes.The stained blood film is examined,and sickle cells are observed on the blood film.Equal volumes of the diluted hemoglobin sample and water are mixed together,and the absorbance of this read on a spectrophotometer.How should the hemoglobin result be determined from the standard calibrated graph?

A) Add the results from the turbid and second dilution together and divide by 2.
B) Multiply the result obtained from the graph on the diluted specimen by 2.
C) Divide the result obtained from the graph on the diluted specimen by 2.
D) There was no reason to perform this second dilution.