Two Species of Tree Frogs That Live Sympatrically in the Eastern

Multiple Choice

Two species of tree frogs that live sympatrically in the eastern United States differ in ploidy: Hyla chrysoscelis is diploid, and Hyla versicolor is tetraploid. The frogs are identical in appearance, but their mating calls, which females use to find mates, differ. Which difference most likely evolved first?

A) polyploidy
B) difference in breeding seasons
C) habitat differentiation
D) female preference for male mating calls