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Which Order of the Following Steps Provides the Most Logical

Question 37

Multiple Choice

Which order of the following steps provides the most logical sequence to follow when splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with a recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes (endonucleases) .
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V. Use ligase to seal plasmid DNA to non-plasmid DNA.


A) II, III, V, IV, I
B) III, II, IV, V, I
C) III, IV, V, I, II
D) IV, V, I, II, III

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