Which of the Following Describes the Best Approach to Making

Multiple Choice

Which of the following describes the best approach to making a gene that contains introns shorter, but remaining functional, for genetic engineering purposes?

A) express in bacteria then a restriction enzyme (endonuclease) to cut the gene into shorter pieces
B) isolate the specific mRNA from the animal then use reverse transcriptase to produce cDNA
C) isolate the specific protein from the animal then use the genetic code to reconstruct DNA from the polypeptide sequence
D) using DNA ligase to put together fragments of the DNA that code for a particular polypeptide